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(A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous <t>TOM20</t> staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4
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(A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous <t>TOM20</t> staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4
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(A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous <t>TOM20</t> staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4
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(A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous <t>TOM20</t> staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4
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Image Search Results


(A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4

Journal: bioRxiv

Article Title: BRET-Based Mitochondrial Subcompartment Localization Biosensors

doi: 10.1101/2025.10.27.684728

Figure Lengend Snippet: (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4

Article Snippet: TOM20-YFP(Nluc) fusion proteins were obtained by fusing the TOM20 coding region (Addgene # 55146) in its c-terminus to YFP or Nluc.

Techniques: Immunofluorescence, Staining

(A) Immunofluorescence images showing mito-biosensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Images showing colocalization of mito-sensors tagged to Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of mito-biosensors in different compartments. (D,E,F) BRET values represent the proximity between CLU-Nluc, IBM-Nluc, and MICU1-Nluc, respectively, and localization sensors (YFP), n =5. (G) Schematic representation of the localization of VDAC1 and localization biosensors in different compartments. (H) BRET values representing the proximity between VDAC1-Nluc and loc-sensors (YFP), n = 4.

Journal: bioRxiv

Article Title: BRET-Based Mitochondrial Subcompartment Localization Biosensors

doi: 10.1101/2025.10.27.684728

Figure Lengend Snippet: (A) Immunofluorescence images showing mito-biosensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Images showing colocalization of mito-sensors tagged to Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of mito-biosensors in different compartments. (D,E,F) BRET values represent the proximity between CLU-Nluc, IBM-Nluc, and MICU1-Nluc, respectively, and localization sensors (YFP), n =5. (G) Schematic representation of the localization of VDAC1 and localization biosensors in different compartments. (H) BRET values representing the proximity between VDAC1-Nluc and loc-sensors (YFP), n = 4.

Article Snippet: TOM20-YFP(Nluc) fusion proteins were obtained by fusing the TOM20 coding region (Addgene # 55146) in its c-terminus to YFP or Nluc.

Techniques: Immunofluorescence, Staining

(A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E, F) BRET values represent the proximity between -MX-Nluc, ATP5F1C-Nluc, and OTC-Nluc, respectively, and localization sensors (YFP). n =5. (G) Schematic representation of the localization of the Sirtuins and MX loc-biosensor in different compartments. (G) BRET values represent the proximity between MX-Nluc, MX-YFP, and sirtuins (YFP), n = 4

Journal: bioRxiv

Article Title: BRET-Based Mitochondrial Subcompartment Localization Biosensors

doi: 10.1101/2025.10.27.684728

Figure Lengend Snippet: (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E, F) BRET values represent the proximity between -MX-Nluc, ATP5F1C-Nluc, and OTC-Nluc, respectively, and localization sensors (YFP). n =5. (G) Schematic representation of the localization of the Sirtuins and MX loc-biosensor in different compartments. (G) BRET values represent the proximity between MX-Nluc, MX-YFP, and sirtuins (YFP), n = 4

Article Snippet: TOM20-YFP(Nluc) fusion proteins were obtained by fusing the TOM20 coding region (Addgene # 55146) in its c-terminus to YFP or Nluc.

Techniques: Immunofluorescence, Staining